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Exosome Research Products

Exosomes are extracellular vesicles secreted from many cells and are found in various body fluids including serum, plasma, saliva and urine. Exosomes consist of lipid bilayer membranes, from 40nm to 100nm in diameter, and contain several proteins and nucleic acid such as microRNA, mRNA and DNA. In recent years, it has been suggested that exosomes may be biomarkers for cancer, diabetes, neurological diseases and various diseases. Exosomes bear specific protein markers of the endosomal pathway, such as tetraspanins (CD63, CD9 and CD81), heat shock proteins (HSP70) and EpCam depending on their cellular origin.

MBLI offers kits, antibodies, tools, and protocols for the isolation of exosomes as well as for characterization.

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Exosomes Featured Image

Method of Exosome Isolation

Major ways of concentrating and isolating exosomes

　　

Ultracentrifugation method

Polymer-based precipitation method

Immunoaffinity method

Method

  • Exosome isolation method utilizing difference of density

  • Exosome precipitation method using polymer

  • Immunoaffinity method using magnetic beads-conjugated antibodies against exosome markers

Advantage

  • Conventional, standard method

  • Suitable for large scale

  • Recover Exosome from variety of samples

  • Low cost

  • Easy operation

  • Recovery of Exosome in a short period of time

  • No specific device, instrument is required

  • Easy operation

  • Recovery of Exosome in a short period of time

  • Few contaminants with high purity

  • Enable to isolate exosomes that express specific marker

Disadvantage

  • Takes time with complicated operation (needs half a day - 2 days)

  • Many contaminants with poor purification

  • Difficult to use multiple samples simultaneously

  • Required for optimized experimental condition to avoid vesicle loss

  • Ultracentrifuge is required

  • Many contaminants with poor purification

  • Need to select the optimal reagents depending on the sample types (e.g. serum, plasma, culture sup)

  • Exosome recovery depends on antibody specificity

MBLI products

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