Exosomes are extracellular vesicles secreted from many cells and are found in various body fluids including serum, plasma, saliva and urine. Exosomes consist of lipid bilayer membranes, from 40nm to 100nm in diameter, and contain several proteins and nucleic acid such as microRNA, mRNA and DNA. In recent years, it has been suggested that exosomes may be biomarkers for cancer, diabetes, neurological diseases and various diseases. Exosomes bear specific protein markers of the endosomal pathway, such as tetraspanins (CD63, CD9 and CD81), heat shock proteins (HSP70) and EpCam depending on their cellular origin.
MBLI offers kits, antibodies, tools, and protocols for the isolation of exosomes as well as for characterization.
ExoCap™ Streptavidin Kit
ExoCap™ kits are designed for exosome isolation and enrichment and are optimized for use with serum, plasma and cell culture supernatants. Exosomes are extracellular vesicles secreted by most cell types that contain marker proteins and microRNAs. ExoCap™ Streptavidin Kit is designed for customized isolation and analysis of exosomes or microvesicles, called Extracellular Vesicles (EVs), using the researcher’s biotinylated molecules such as antibodies against exosome surface marker proteins. ExoCap™ uses functionalized Magnosphere™, magnetic micro-particles coated with JSR Life Sciences proprietary hydrophilic polymer to decrease non-specific binding.