Smart IP: Fast and Effective IP
Smart-IP is a line of highly specific tag antibodies conjugated to magnetic beads or magnetic agarose beads designed for faster immunoprecipitation. Smart-IP improves efficiency by reducing time-consuming steps such as centrifugation. The magnetic beads and magnetic agarose beads can be easily isolated using a magnetic rack. The supernatant can also be extracted with minimal sample loss resulting in high yields of your target position.
- High protein recovery
- No pre-clear step
- Complete removal of supernatant
- Ready to use
Description of Each Conjugate
| Magnetic Beads | MAGNETIC AGAROSE | AGAROSE | |
|---|---|---|---|
|
Applications
|
IP
|
IP, Purification
|
IP, Purification
|
|
Bead Diameter
|
1.5 μm
|
50 μm
|
100 μm
|
|
Schematic Diagram
|
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Product Images
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Pros
|
Almost no sample lossEasy to see the beadsEasy to disperse, can be used for screening
|
Large amount of IgG/per beadsLess sample lossEasy to see the beads
|
Large amount of IgG/per beadsNo need of magnetic rackLower cost
|
|
Cons
|
Small amount of IgG/per beadsMagnetic rack will be needed
|
Magnetic rack will be needed
|
Might lose sample during the washHard to see the gel
|
Product List by Epitope Tag
| EPITOPE TAG | Clonality | CLONE NUMBER | ISOTYPE | SIZES | CODE NO.: CONJUGATE TO MAGNETIC BEADS | CODE NO.: CONJUGATED TO MAGNETIC AGAROSE | CODE NO.: CONJUGATED TO AGARAOSE |
|---|---|---|---|---|---|---|---|
|
DDDDK
|
Monoclonal
|
FLA-1
|
Mouse IgG2ak
|
1 mL
|
|||
|
GFP
|
Monoclonal
|
RQ2
|
Rat IgG2ak
|
1 mL
|
|||
|
HA
|
Monoclonal
|
5D8
|
Mouse IgG1k
|
1 mL
|
|||
|
HA
|
Monoclonal
|
TANA2
|
Mouse IgG2bk
|
1 mL
|
|
||
|
His
|
Monoclonal
|
OGHis
|
Mouse IgG2ak
|
1 mL
|
|||
|
Myc
|
Monoclonal
|
PL14
|
Mouse IgG1k
|
1 mL
|
|||
|
RFP
|
Monoclonal
|
3G5
|
Mouse IgG1k
|
1 mL
|
|||
|
V5
|
Monoclonal
|
1H6
|
Mouse IgG2ak
|
1 mL
|
Trial sizes available, contact us for details.
References:
- Cai, L., et al., J. Biol. Chem. 286, 35915-35921 (2011)
- Sato, Y., et al., J. Biol. Chem. 284, 11873-11881 (2009)
- Sakurai, T., et al., J. Cell Biol. 183, 339-352 (2008)