Unlike other ubiquitin-like proteins, the ubiquitin-like protein Atg12 has a C-terminal glycine, which protects it from processing. Atg12 is conjugated to the substrate Atg5 by Atg7 (an E1-like protein, and as seen in the conjugation of Atg8 to PE) and Atg10 (an E2-like protein). The Atg12-Atg5 conjugate forms a complex with Atg16. Self-oligomerization of Atg16 results in a multimer of the Atg12-Atg5-Atg16 complex. Although its functions remain unknown, this complex is shown to accumulate on the pre-autophagosomal structure (PAS) in yeast and to play an essential role in autophagosome formation.
Anti-Atg5 Antibodies: Code No. PM050 (Polyclonal) & M153-3 (Monoclonal)
Anti-Atg12 (Human) antibody: Code No. M154-3 (Monoclonal)
Anti-Atg10 (Human) antibody: Code No. M151-3 (Monoclonal)
Anti-Atg7 (Human) antibody: Code No. PM039 (Polyclonal)
Learn more about other popular Autophagy targets and products
Autophagy
Autophagy is a cellular self-digestion process for the purpose of providing nutrients to allow for cell survival during stress conditions.
LC3/ATG8
LC3 is most actively studied and frequently used as a mammalian autophagy marker.
P62/SQSTM1
62/SQSTM1 is thought to be a substrate that binds directly to LC3 causing its selective degradation by autophagy.
ATG16L
Atg16L forms an approximately 800 kDa complex with the Atg12-Atg5 conjugate.
ULK1
ULK1/Atg1 is a serine/threonine protein kinase essential for autophagy.
ATG14
Atg14L forms a class III PI3K complex with Beclin1, Vps34 and p150.
Atg9
Atg9 is thought to be involved in the early phage of PAS formation.
